Plasma extracellular microRNAs are related to AIDS/cerebral toxoplasmosis co‐infection. (28th January 2020)

• Record Type: Journal Article
• Authors: Pereira, Ingrid de Siqueira; Maia, Marta Marques; da Cruz, Allecineia Bispo; Telles, João Paulo Marochi; Vidal, Jose Ernesto; Gava, Ricardo; Meira‐Strejevitch, Cristina Silva; Pereira‐Chioccola, Vera Lucia
• Is Part Of: Parasite immunology. Volume 42:Number 4(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-01-28
• Subjects: cerebral toxoplasmosis -- cytokine regulation -- extracellular miRNA -- gene expression; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12696
• Languages: English
• ISSNs: 0141-9838

Abstract

Abstract: This study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co‐infection (CT/HIV) biomarkers. miR‐155‐5p, miR‐146a‐5p, miR‐21‐5p, miR‐125b‐5p and miR‐29c‐3p were tested in 79 plasma divided into groups: 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmosis (AT); and 20 individuals seronegative for toxoplasmosis (NC). From each was collected peripheral blood/EDTA for laboratory diagnosis. Blood cells for DNA extractions (molecular diagnosis), plasma for RNA extractions (gene expression) and ELISA (serological diagnosis). miRNA expression was performed by qPCR, and values were expressed in Relative Quantification (RQ). Among the five miRNAs, miR‐21‐5p and miR‐146a‐5p were up‐expressed in CT/HIV group when compared with AT and NC groups. RQ means for miR‐21‐5p and miR‐146a‐5p in CT/HIV group were 3.829 and 2.500, while in AT group, were 1.815 and 1.661, respectively. Differences between 3 groups were statistically significant (Kruskal‐Wallis ANOVA test), as well as CT/HIV and AT groups (Mann‐Whitney test). Plasma of CT/HIV and AT groups expressed similar levels of miR‐29c‐3p, miR‐155‐5p and miR‐125b‐5p. As NC group was different of CT/HIV and AT groups, differences between three groups were statistically significant (Kruskal‐Wallis ANOVA test). No difference was shown between CT/HIV and AT groups (Mann‐Whitney test). These results suggest the host miRNAs modulation by Toxoplasma gondii .