Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization. (20th March 2020)

• Record Type: Journal Article
• Authors: Wei, Yan; Shi, Miusi; Zhang, Jinglun; Zhang, Xiaoxin; Shen, Kailun; Wang, Rui; Miron, Richard J.; Xiao, Yin; Zhang, Yufeng
• Is Part Of: Advanced functional materials. Volume 30:Number 21(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-20
• Subjects: biomimics -- biomineralization -- bone regeneration -- extracellular matrix -- extracellular vesicles; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202000015
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Restoring extracellular matrix (ECM) with supportive and osteoinductive abilities is of great significance for bone tissue regeneration. Current approaches involving cell‐based scaffolds or nanoparticle‐modified biomimic‐ECM have been met with additional biosafety concerns. Herein, the natural biomineralization process is first analyzed and is found that mesenchymal stem cells‐derived extracellular vesicles (EVs) from early and late stages of osteoinduction play different roles during the mineralization process. The functional EVs hierarchically with blood‐derived autohydrogel (AH) are then incorporated to form an osteoinductive biomimetic extracellular matrix (BECM). The alkaline phosphatase‐rich EVs are released from the outer layers to induce osteoblast differentiation during early stages. Thereafter, as the degradation of AH occurred, calcium/phosphorus (Ca/P)‐rich EVs are liberated to promote the nucleation of extracellular mineral crystals. Additionally, BECM contains considerable collagen fibrils that provide additional nucleation sites for crystallites deposition, thus reaching self‐mineralization in situ. In conclusion, this research provides a promising, versatile mineralization‐instructive platform to tackle the challenges faced in bone‐tissue engineering. Abstract : Restoring extracellular matrix with supportive and osteoinductive abilities is of great significance for bone tissue regeneration. Autologous blood‐derived hydrogels and functional extracellular vesicles (EVs) construct a biomimetic extracellular matrix (BECM). EVs with different properties are sustainingly released during mineralization process. In addition, collagen fibrils in BECM provide additional nucleation sites for crystallites deposition, thus reaching self‐mineralization in situ.