Development of small‐scale models to understand the impact of continuous downstream bioprocessing on integrated virus filtration. (3rd February 2020)

• Record Type: Journal Article
• Authors: Lute, Scott; Kozaili, Julie; Johnson, Sarah; Kobayashi, Kazuya; Strauss, Daniel
• Is Part Of: Biotechnology progress. Volume 36:Number 3(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-03
• Subjects: biotechnology -- continuous manufacturing -- small‐scale models -- viral filtration -- virus clearance; Biotechnology -- Periodicals; Food industry and trade -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/btpr.2962
• Languages: English
• ISSNs: 8756-7938

Abstract

Abstract: We designed small‐scale virus filtration models to investigate the impact of the extended process times and dynamic product streams present in continuous manufacturing. Our data show that the Planova 20N and BioEX virus filters are capable of effectively removing bacteriophage PP7 (>4 log) when run continuously for up to 4 days. Additionally, both Planova 20N and BioEX filters were able to successfully process a mock elution peak of increased protein, salt, and bacteriophage concentrations with only an increase in filtration pressure observed during the higher protein concentration peak. These experiments demonstrated that small‐scale viral clearance studies can be designed to model a continuous virus filtration step with specific process parameters.