Wuchereria bancrofti macrophage migration inhibitory factor‐2 (rWbaMIF‐2) ameliorates experimental colitis. (6th February 2020)

• Record Type: Journal Article
• Authors: Ramani, Shriram; Chauhan, Nikhil; Khatri, Vishal; Vitali, Connie; Kalyanasundaram, Ramaswamy
• Is Part Of: Parasite immunology. Volume 42:Number 4(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-06
• Subjects: B1 cells -- Brugia malayi -- colitis -- DSS -- macrophage migration inhibitory factor -- T regulatory cells -- Wuchereria bancrofti; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12698
• Languages: English
• ISSNs: 0141-9838

Abstract

Abstract: Immunomodulatory molecules produced by helminth parasites are receiving much attention recently as novel therapeutic agents for inflammation and autoimmune diseases. In this study, we show that macrophage migration inhibitory factor (MIF) homologue from the filarial parasite, Wuchereria bancrofti (r Wba MIF‐2), can suppress inflammation in a dextran sulphate sodium salt (DSS)‐induced colitis model. The disease activity index (DAI) in DSS given mice showed loss of body weight and bloody diarrhoea. At autopsy, colon of these mice showed severe inflammation and reduced length. Administration of r Wba MIF‐2 significantly reduced the DAI in DSS‐induced colitis mice. r Wba MIF‐2‐treated mice had no blood in the stools, and their colon length was similar to the normal colon with minimal inflammation and histological changes. Pro‐inflammatory cytokine genes (TNF‐α, IL‐6, IFN‐γ, IL‐1β, IL‐17A and NOS2) were downregulated in the colon tissue and peritoneal macrophages of r Wba MIF‐2‐treated mice. However, there were significant increases in IL‐10‐producing Treg and B1 cells in the colon and peritoneal cavity of r Wba MIF‐2‐treated mice. These findings suggested that r Wba MIF‐2 treatment significantly ameliorated the clinical symptoms, inflammation and colon pathology in DSS given mice. This immunomodulatory effect of r Wba MIF‐2 appeared to be by promoting the infiltration of Treg cells into the colon.