Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation. (13th March 2020)

• Record Type: Journal Article
• Authors: Hiraoka, Haruka; Nakano, Tadashi; Kuwana, Satoshi; Fukuzawa, Masashi; Hirano, Yasuhiro; Ueda, Masahiro; Haraguchi, Tokuko; Hiraoka, Yasushi
• Is Part Of: Genes to cells. Volume 25:Number 5(2020)
• Page Start: 312
• Page End: 326
• Publication Date: 2020-03-13
• Subjects: ATeam -- ATP -- ATP sensor probe -- cell differentiation -- glycolysis -- live‐cell imaging -- MaLion -- metabolism -- mitochondria -- TCA cycle; Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12763
• Languages: English
• ISSNs: 1356-9597

Abstract

Abstract: Multicellular organisms contain various differentiated cells. Fate determination of these cells remains a fundamental issue. The cellular slime mold Dictyostelium discoideum is a useful model organism for studying differentiation; it proliferates as single cells in nutrient‐rich conditions, which aggregate into a multicellular body upon starvation, subsequently differentiating into stalk cells or spores. The fates of these cells can be predicted in the vegetative phase: Cells expressing higher and lower levels of omt12 differentiate into stalk cells and spores, respectively. However, omt12 is merely a marker gene and changes in its expression do not influence the cell fate, and determinant factors remain unknown. In this study, we analyzed cell fate determinants in the stalk‐destined and spore‐destined cells that were sorted based on omt12 expression. Luciferase assay demonstrated higher levels of intracellular ATP in the stalk‐destined cells than in the spore‐destined cells. Live‐cell observation during development using ATP sensor probes revealed that cells with higher ATP levels differentiated into stalk cells. Furthermore, reducing the ATP level by treating with an inhibitor of ATP production changed the differentiation fates of the stalk‐destined cells to spores. These results suggest that intracellular ATP levels influence cell fates in D. discoideum differentiation. Abstract : We analyzed differentiation fate determinants in the cellular slime mold Dictyostelium discoideum . Vegetable cells were sorted by cell sorter to collect stalk‐destined and spore‐destined cells, and ATP levels in those cells were visualized during development. Our results suggest that intracellular ATP levels act as an intrinsic cell fate determinant in D. discoideum differentiation.