A Laccase/Chitosan‐Lambda‐Carrageenan Based Voltammetric Biosensor for Phenolic Compound Detection. Issue 4 (5th November 2019)

• Record Type: Journal Article
• Authors: Chakroun Galai, Hajer; Rassas, Ilhem; Namour, Philippe; Bonhomme, Anne; Raimondi, Gaetan; Besbes Hentati, Salma; Jaffrezic‐Renault, Nicole
• Is Part Of: Electroanalysis. Volume 32:Issue 4(2020)
• Page Start: 732
• Page End: 740
• Publication Date: 2019-11-05
• Subjects: biosensor -- laccase -- chitosan -- carrageenan -- catechol; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201900531
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: In this contribution, a new concept of voltammetric catechol biosensor, based on the encapsulation of laccase (LAC) in a chitosan+lambda‐carrageenan (CHIT+CAR) polyelectrolyte complex (PEC) employing a simple coacervation process is presented. Chitosan (CHIT) was prepared from α‐chitin extracted from shrimp shells and lambda‐carrageenan (CAR) was extracted from red algae, both polysaccharides and PEC being characterized using FTIR spectrometry and electrochemistry. Cyclic voltammetry was utilized to determine the analytical features of the laccase (LAC) biosensor for catechol detection. The linear range was from 10 −20 M to 10 −14 M with a sensitivity of 1.55 mA/p[catechol] and a limit of detection of 3×10 −21 M.The laccase biosensor exhibits good repeatability (RSD 2.38 %) and stability (four weeks). The developed biosensor was tested by applying it to the evaluation of the total polyphenolic content in natural oil samples. Abstract :