Expression of small RNAs of Bordetella pertussis colonizing murine tracheas. (13th May 2020)

• Record Type: Journal Article
• Authors: Hiramatsu, Yukihiro; Suzuki, Koichiro; Motooka, Daisuke; Nakamura, Shota; Horiguchi, Yasuhiko
• Is Part Of: Microbiology and immunology. Volume 64:Number 6(2020)
• Page Start: 469
• Page End: 475
• Publication Date: 2020-05-13
• Subjects: Bordetella pertussis -- BvgAS -- gene regulatory system -- in vivo RNA sequencing -- small RNA; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12791
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: We performed RNA sequencing on Bordetella pertussis, the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressed in vivo than in vitro . Moreover, the expression of eight sRNAs was not regulated by the BvgAS two‐component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS‐independent gene regulatory system involving the sRNAs that is active during B. pertussis infection.