Estimation of Protein and Fatty Acid Composition in Shell‐Intact Cottonseed by Near Infrared Reflectance Spectroscopy. (1st December 2019)

• Record Type: Journal Article
• Authors: Wang, Qingkang; Xing, Huixian; Liu, Xiangliu; Mao, Lili; Wei, Ze; Zhang, Haijun; Wang, Liyuan; Wang, Haoran; Saeed, Muhammad; Zhang, Guihua; Song, Xianliang; Sun, Xue‐Zhen; Yuan, Yanchao
• Is Part Of: Journal of the American Oil Chemists' Society. Volume 97:Number 4(2020)
• Page Start: 331
• Page End: 340
• Publication Date: 2019-12-01
• Subjects: Shell‐intact cottonseed -- Near infrared reflectance spectroscopy (NIRS) -- Protein content -- Seed total fatty acid (STA) content -- Cottonseed kernel total fatty acid (KTA) content -- Fatty acid composition; Oils and fats -- Periodicals; Soap -- Periodicals; Fats -- Periodicals; Food-Processing Industry -- Periodicals; Acides gras -- Périodiques; Aliments -- Industrie et commerce -- Périodiques; Huiles et graisses -- Périodiques; Savon -- Périodiques; Oliën; Chemie; Oils and fats; Soap; Periodicals; 547.77
• DOI: 10.1002/aocs.12312
• Languages: English
• ISSNs: 0003-021X

Abstract

Abstract: Rapid and accurate analysis of cottonseed protein content and the composition of fatty acids (especially, saturated fatty acids) is often required in cotton production and breeding programs. This study aimed to establish a set of effective estimation models for these parameters. Near infrared reflectance spectroscopy (NIRS) calibration equations using partial least‐squares regression for protein concentration, oil concentration, and five fatty acids of shell‐intact cottonseeds were established based on 90 varieties, and the prediction abilities of the calibration models were verified using 45 other varieties. The prediction abilities of the NIRS calibration equations were basically consistent with external validation results. Each equation was assessed based on the ratio of performance to deviation (RPDp ). Protein content and seed total fatty acid (STA) content had high RPDp values (3.687 and 3.530, respectively), whereas cottonseed kernel total fatty acid (KTA) content, linoleic acid (18:2), stearic acid (18:0), myristic acid (14:0), and palmitic acid (16:0) exhibited relatively high RPDp (2.866, 2.836, 2.697, 2.676, and 2.506, respectively). The calibration model for oleic acid (18:1) had a low RPDp (1.945). The results indicated that NIRS can be used to rapidly determine contents of STA, KTA, protein, stearic acid (18:0), myristic acid (14:0), and palmitic acid (16:0) in shell‐intact cottonseed.