Flow Homogenization Enables a Massively Parallel Fluidic Design for High‐Throughput and Multiplexed Cell Isolation. Issue 5 (18th March 2020)

• Record Type: Journal Article
• Authors: Ooi, Chinchun; Earhart, Christopher M.; Hughes, Casey E.; Lee, Jung‐Rok; Wong, Dawson J.; Wilson, Robert J.; Rohatgi, Rajat; Wang, Shan X.
• Is Part Of: Advanced materials technologies. Volume 5:Issue 5(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-18
• Subjects: flow homogenization -- magnetic separation -- microfluidics -- multiplexed cell separation -- rare cell isolation; Materials science -- Periodicals; Technological innovations -- Periodicals; Materials science; Technological innovations; Periodicals; 620.1105
• DOI: 10.1002/admt.201900960
• Languages: English
• ISSNs: 2365-709X

Abstract

Abstract: Microfluidic devices are widely used for applications such as cell isolation. Currently, the most common method to improve throughput for microfluidic devices involves fabrication of multiple, identical channels in parallel. However, this "numbering up" only occurs in one dimension, thereby limiting gains in volumetric throughput. In contrast, macrofluidic devices permit high volumetric flow rates but lack the finer control of microfluidics. Here, it is demonstrated how a micropore array design enables flow homogenization across a magnetic cell capture device, thus creating a massively parallel series of microscale flow channels with consistent fluidic and magnetic properties, regardless of spatial location. This design enables scaling in two dimensions, allowing flow rates exceeding 100 mL h −1 while maintaining >90% capture efficiencies of spiked lung cancer cells from blood in a simulated circulating tumor cell system. Additionally, this design facilitates modularity in operation, which is demonstrated by combining two different devices in tandem for multiplexed cell separation in a single pass with no additional cell losses from processing. Abstract : Multiplexed and high‐throughput cell separation can be immensely useful in areas such as circulating tumor cell or circulating nucleated fetal cell isolation and analysis. This work details the physical principle behind a micropore design that utilizes a flow homogenization phenomenon to rapidly and simply separate a heterogenous cell population into its subpopulations in a single pass with minimal processing losses.