A Smart Nanotherapeutic Agent for in vitro and in vivo Reversal of Heavy‐Metal‐Induced Causality: Key Information from Optical Spectroscopy. (27th December 2019)

• Record Type: Journal Article
• Authors: Adhikari, Aniruddha; Biswas, Pritam; Mondal, Susmita; Das, Monojit; Darbar, Soumendra; Hameed, Ahmed M.; Alharbi, Ahmed; Ahmed, Saleh A.; Sankar Bhattacharya, Siddhartha; Pal, Debasish; Kumar Pal, Samir
• Is Part Of: ChemMedChem. Volume 15:Number 5(2020)
• Page Start: 420
• Page End: 429
• Publication Date: 2019-12-27
• Subjects: Heavy metal toxicity -- Nanomedicine -- Surface functionalized Mn3O4 nanoparticles -- Chelation -- Lead toxicity; Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1002/cmdc.201900543
• Languages: English
• ISSNs: 1860-7179

Abstract

Abstract: Human exposure to heavy metals can cause a variety of life‐threatening disorders, affecting almost every organ of the body, including the nervous, circulatory, cardiac, excretory, and hepatic systems. The presence of heavy metal (cause) and induced oxidative stress (effect) are both responsible for the observed toxic effects. The conventional and effective way to combat heavy metal overload diseases is through use of metal chelators. However, they possess several side effects and most importantly they fail to manage the entire causality. In this study, we introduce citrate‐functionalized Mn3 O4 nanoparticles (C−Mn3 O4 NPs) as an efficient chelating agent for treatment of heavy metal overload diseases. By means of UV/Vis absorbance and steady‐state fluorescence spectroscopic techniques we investigated the efficacy of the NPs in chelation of a model heavy metal, lead (Pb). We also explored the retention of antioxidant properties of the Pb‐chelated C−Mn3 O4 NPs using a UV/Vis‐assisted DPPH assay. Through CD spectroscopic studies we established that the NPs can reverse the Pb‐induced structural modifications of biological macromolecules. We also studied the in vivo efficacy of NPs in Pb‐intoxicated C57BL/6j mice. The NPs were not only able to mobilize the Pb from various organs through chelation, but also saved the organs from oxidative damage. Thus, the C−Mn3 O4 NPs could be an effective nanotherapeutic agent for complete reversal of heavy‐metal‐induced toxicity through chelation of the heavy metal and healing of the associated oxidative stress. Abstract : Citrate‐functionalized Mn3 O4 nanoparticles (C−Mn3 O4 NPs) can efficiently chelate lead (Pb 2+ ) ions as well as scavenge reactive oxygen species, thereby acting as potential detoxifying agents of heavy metals. In animals the C−Mn3 O4 NPs are not only able to mobilize Pb 2+ ions from various organs through chelation, but were also found to save the organs from oxidative damage.