Genetic characterization of anemone‐type chrysanthemum (Chrysanthemum morifolium) using floral morphology and SRAP markers. (19th November 2019)

• Record Type: Journal Article
• Authors: Yang, Xiaodong; Ao, Ni; Qu, Yixin; Wu, Yangyang; Su, Jiangshuo; Ding, Lian; Chen, Sumei; Jiang, Jiafu; Guan, Zhiyong; Chen, Fadi; Fang, Weimin; Zhang, Fei
• Editors: Debener, Thomas
• Is Part Of: Plant breeding. Volume 139:Number 2(2020)
• Page Start: 419
• Page End: 427
• Publication Date: 2019-11-19
• Subjects: chrysanthemum -- flower type -- genetic diversity -- molecular markers; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12781
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: An appreciation of the genetic diversity retained in the gene pool of anemone‐type chrysanthemums is needed to drive further genetic improvement of this commercially important ornamental species. Here, a panel of 67 accessions was characterized with respect to both floral morphology, assessed using a set of 12 inflorescence‐associated traits, and to genotype, using sequence‐related amplified polymorphism (SRAP) fingerprinting. Abundant variation and a high level of broad‐sense heritability (>88%) were revealed for all traits except number of tubular floret lobes. Of the possible 66 pairwise inter‐trait combinations, 37 proved to be significantly correlated. A principal component analysis of the trait data showed that the first four principal components explained ~78% of the variance; meanwhile a hierarchical clustering analysis revealed a number of discrete groups reflecting the various cultivated types, but was not predictive of provenance. The SRAP genotyping employed 22 primer combinations and generated 238 informative fragments. A moderately high level of the Jaccard's similarity coefficient (mean 0.67) was calculated for the examined panel. A hierarchical clustering analysis based on the genotypic data revealed four groups, which were clearly reflective of cultivated type; moreover, a comparison of the within‐cluster means for each of the two key anemone‐related traits—tubular floret length and tubular floret length/style length—showed that the genotype‐based clusterings were associated with the anemone levels, albeit imperfectively. A principal coordinate analysis showed that the first two coordinates accounted for about 14% of the total variation. In summary, the present study adds new understanding of the genetic diversity present in the anemone‐type chrysanthemum gene pool, and the findings will lay the foundation for implementing a genotype/phenotype association strategy to reveal the genetic and molecular basis of floral form in this valuable ornamental species.