1H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase. Issue 4 (6th January 2020)

• Record Type: Journal Article
• Authors: Becerra‐Martínez, Elvia; Pacheco‐Hernández, Yesenia; Lozoya‐Gloria, Edmundo; Betancourt‐Jiménez, Martha G.; Hidalgo‐Martínez, Diego; Zepeda‐Vallejo, Luis G.; Villa‐Ruano, Nemesio
• Is Part Of: Phytochemical analysis. Volume 31:Issue 4(2020)
• Page Start: 480
• Page End: 487
• Publication Date: 2020-01-06
• Subjects: tobacco -- metabolomics profiling -- antioxidants -- antitumourals -- phytoalexins; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2911
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Nicotiana tabacum is a plant model intensively used in the bio‐engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non‐targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available. Objective: To compare the proton nuclear magnetic resonance ( 1 H‐NMR) metabolomics profiling of a recombinant Nicotiana tabacum strain containing a promoter of a sesquiterpene cyclase from Capsicum annuum driving GUS expression, versus wild‐type samples. Methodology The non‐targeted 1 H‐NMR metabolome was obtained and processed by principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS‐DA). The differential metabolites were quantified by quantitative NMR. Results: PCA and OPLS‐DA revealed 37 metabolites including 16 discriminant compounds for transgenic samples. Ethanol (0.4 mg g ‐1 ), the main differential compound, was exclusively detected in transgenic tobacco; however, high levels of formate (0.28 mg g ‐1 ) and acetate (0.3 mg g ‐1 ) were simultaneously observed in the same group of samples. Cembratriene‐4, 6‐diol, an antitumour and neuroprotective compound, and capsidiol, a known phytoalexin, increased by about 30% in transgenic samples. In addition, the endogenous levels of the antioxidant caffeoylquinic acid isomers increased by 50% in comparison to those of wild‐type tobaccos. Conclusion: Our results support the occurrence of metabolic differences between wild type and transgenic tobacco containing a promoter of a Capsicum sesquiterpene cyclase gene. Interestingly, the recombinant transgenic strain studied accumulated high amounts of added value compounds with biological activity. Abstract : Proton nuclear magnetic resonance ( 1 H‐NMR) metabolomics revealed an accumulation of bioactive compounds in transgenic tobacco.