Bioactive peptides attenuate cardiac apoptosis in spontaneously hypertensive rat hearts through activation of autophagy and mitochondrial biogenesis pathway. Issue 7 (5th March 2020)

• Record Type: Journal Article
• Authors: Lin, Wan Teng; Nithiyanantham, Srinivasan; Hsieh, Dennis Jine‐Yuan; Chen, Ray‐Jade; Day, Cecilia‐Hsuan; Liao, Jia Ying; Kuo, Chia‐Hua; Mahalakshmi, B.; Kuo, Wei‐Wen; Huang, Chih‐Yang
• Is Part Of: Environmental toxicology. Volume 35:Issue 7(2020)
• Page Start: 804
• Page End: 810
• Publication Date: 2020-03-05
• Subjects: apoptosis -- autophagy -- bioactive peptides -- mitochondrial biogenesis -- SHR; Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.22916
• Languages: English
• ISSNs: 1520-4081

Abstract

Abstract: Alcalase potato protein hydrolysate (APPH) might have a very important role in therapeutic effects. This study aims to examine the beneficial effects of bioactive peptides (DIKTNKPVIF [DI] and IF) from APPH supplement in the regulation of cardiac apoptosis, autophagy, and mitochondrial biogenesis pathway in spontaneously hypertensive rats (SHR). We have investigated ejection fraction, fractional shortening, Tunel assay, apoptosis, autophagy, and mitochondrial biogenesis pathway marker expression to show the efficacy of bioactive peptides in an SHR model. Bioactive peptides significantly upregulate ejection fraction and fractional shortening in SHR rats. SHR rats exhibited higher protein expression of apoptotic markers such as BAD, cytochrome c, and caspase 3. Finally, the bioactive peptides upregulate survival proteins (p‐AKT/p‐PI3K), autophagy (Beclin1/LC3B), and mitochondrial biogenesis (p‐AMPKα/SIRT1/PGC1α/p‐Foxo3a/Nrf2/CREB) marker expressions compared with the SHR groups. In summary, the bioactive peptides protect the heart tissues through the activation of autophagy and mitochondrial biogenesis pathway and thereby attenuate cardiac apoptosis in a spontaneously hypertensive rat model.