A competition between hydrophobic and electrostatic interactions in protein–ligand systems. Binding of heterogeneously halogenated benzotriazoles by the catalytic subunit of human protein kinase CK2. Issue 6 (12th March 2020)

• Record Type: Journal Article
• Authors: Kasperowicz, Sławomir; Marzec, Ewa; Maciejewska, Agnieszka M.; Trzybiński, Damian; Bretner, Maria; Woźniak, Krzysztof; Poznański, Jarosław; Mieczkowska, Kinga
• Other Names: Poznanski Jarek guestEditor.; Fabbro Doriano guestEditor.
• Is Part Of: IUBMB life. Volume 72:Issue 6(2020)
• Page Start: 1211
• Page End: 1219
• Publication Date: 2020-03-12
• Subjects: bromobenzotriazoles -- chlorobenzotriazoles -- chromatographic hydrophobicity index -- crystal structure -- halogenated benzotriazoles -- human protein kinase CK2 -- thermal shift assay; Biochemistry -- Periodicals; Molecular biology -- Periodicals; 572.8
• DOI: 10.1002/iub.2271
• Languages: English
• ISSNs: 1521-6543

Abstract

Abstract: A series of chlorine‐substituted benzotriazole derivatives, representing all possible substitution patterns of halogen atoms attached to the benzotriazole benzene ring, were synthetized as potential inhibitors of human protein kinase CK2. Basic ADME parameters for the free solutes (hydrophobicity, electronic properties) together with their binding affinity to the catalytic subunit of protein kinase CK2 were determined with reverse‐phase HPLC, spectrophotometric titration, and Thermal Shift Assay Method, respectively. The analysis of position‐dependent thermodynamic contribution of a chlorine atom attached to the benzotriazole ring confirmed the previous observation for brominated benzotriazoles, in which substitution at positions 5 and 6 with bromine was found crucial for ligand binding. In all tested halogenated benzotriazoles the replacement of Br with Cl decreases the hydrophobicity, while the electronic properties remain virtually unaffected. Supramolecular architecture identified in the just resolved crystal structures of three of the four possible dichloro‐benzotriazoles shows how substitution distant from the triazole ring affects the pattern of intermolecular interactions. Summarizing, the benzotriazole benzene ring substitution pattern has been identified as the main driver of ligand binding, predominating the non‐specific hydrophobic effect.