Traceable Impedance-Based Dispensing and Cloning of Living Single Cells. (June 2020)

• Record Type: Journal Article
• Authors: Muller, Georges; Bonzon, David; Claudinot, Stéphanie; Rochat, Ariane; Renaud, Philippe; Barrandon, Yann
• Is Part Of: SLAS technology. Volume 25:Number 3(2020)
• Page Start: 215
• Page End: 221
• Publication Date: 2020-06
• Subjects: automated biology -- microfluidics -- microtechnology -- systems -- engineering; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2472630320905574
• Languages: English
• ISSNs: 2472-6303

Abstract

Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.