Human iPSC‐derived microglia: A growing toolset to study the brain's innate immune cells. Issue 4 (11th January 2020)

• Record Type: Journal Article
• Authors: Hasselmann, Jonathan; Blurton‐Jones, Mathew
• Other Names: Kettenmann Helmut guestEditor.; Ransom Bruce R. guestEditor.
• Is Part Of: Glia. Volume 68:Issue 4(2020)
• Page Start: 721
• Page End: 739
• Publication Date: 2020-01-11
• Subjects: cerebral organoids -- chimeric -- induced pluripotent stem cells -- microglia -- microglia‐like cells -- xenotransplantation; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23781
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Recent advances in the generation of microglia from human induced pluripotent stem cells (iPSCs) have provided exciting new approaches to examine and decipher the biology of microglia. As these techniques continue to evolve to encompass more complex in situ and in vivo paradigms, so too have they begun to yield novel scientific insight into the genetics and function of human microglia. As such, researchers now have access to a toolset comprised of three unique "flavors" of iPSC‐derived microglia: in vitro microglia (iMGs), organoid microglia (oMGs), and xenotransplanted microglia (xMGs). The goal of this review is to discuss the variety of research applications that each of these techniques enables and to highlight recent discoveries that these methods have begun to uncover. By presenting the research paradigms in which each model has been successful, as well as the key benefits and limitations of each approach, it is our hope that this review will help interested researchers to incorporate these techniques into their studies, collectively advancing our understanding of human microglia biology. Abstract : Stem cell technology is increasingly being used to model human microglia. In vitro, in situ, and in vivo techniques exist to study human iPSC‐derived microglia. Application of these techniques has begun to reveal novel aspects of microglia biology.