Production of butanol and polyhydroxyalkanoate from industrial waste by Clostridium beijerinckii ASU10. (2nd April 2019)

• Record Type: Journal Article
• Authors: Hassan, Elhagag Ahmed; Abd‐Alla, Mohamed Hemida; Zohri, Abdel‐Naser Ahmed; Ragaey, Marwa M.; Ali, Shimaa Mohamed
• Is Part Of: International journal of energy research. Volume 43:Number 8(2019)
• Page Start: 3640
• Page End: 3652
• Publication Date: 2019-04-02
• Subjects: acetone‐butanol‐ethanol fermentation -- biofuel -- biohydrogen -- Clostridium beijerinckii ASU10 -- polyhydroxyalkanoates; Power resources -- Periodicals; Power (Mechanics) -- Periodicals; Power resources -- Research -- Periodicals; 621.042
• DOI: 10.1002/er.4514
• Languages: English
• ISSNs: 0363-907X

Abstract

Summary: This study demonstrated a biotechnological approach for simultaneous production of low‐cost H2, liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium ( Clostridium beijerinckii ) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H2 h −1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L −1, respectively. ABE productivity (g L −1 h −1 ) was 0.0486 and 0.0564 with a yield rate (g g −1 ) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials. Abstract : A potent Clostridium beijerinckii ASU10 (KF372577) is considered a promising strain for low‐cost PHA production as intracellular stored inclusion bodies integrated with the production of high‐energy biofuel and other valuable compounds from utilization of organic waste materials. C beijerinckii ASU10 exhibited considerable performance in hydrogen production from glycerol (5.1 ml H2 h −1 ) and sugarcane molasses (11 ml H2 h −1 ), and the generation of acetone‐butanol‐ethanol (ABE) from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L −1, respectively. Additionally, the PHA accumulation from glycerol and sugarcane molasses was 84.37% and 37.94% of the dried bacterial biomass, respectively, which was confirmed by the ultrathin section (TEM) of C beijerinckii ASU10 showed more densely accumulated PHA granules. Furthermore, GC‐MS analysis confirmed that the PHAs obtained from fermentation included 3‐hydroxybutyrate and 3‐hydroxyoctanoate as the main constituents.