Characterization of three heat shock protein 70 genes from Liriomyza trifolii and expression during thermal stress and insect development. Issue 2 (10th May 2018)

• Record Type: Journal Article
• Authors: Chang, Y.-W.; Zhang, X.-X.; Chen, J.-Y.; Lu, M.-X.; Gong, W.-R.; Du, Y.-Z.
• Is Part Of: Bulletin of entomological research. Volume 109:Issue 2(2019)
• Page Start: 150
• Page End: 159
• Publication Date: 2018-05-10
• Subjects: Liriomyza trifolii, -- heat shock protein 70, -- thermal stress, -- developmental stage, -- expression profile; Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485318000354
• Languages: English
• ISSNs: 0007-4853

Abstract

Abstract: Heat shock proteins (HSPs) participate in diverse physiological processes in insects, and HSP70 is one of the most highly conserved proteins in the HSP family. In this study, full-length cDNAs of three HSP70 genes ( Lthsc70, Lthsp701, and Lthsp702 ) were cloned and characterized from Liriomyza trifolii, an important invasive pest of vegetable crops and horticultural crops worldwide. These three HSP70s exhibited signature sequences and motifs that are typical of the HSP70 family. The expression patterns of the three Lthsp70 s during temperature stress and in different insect development stages were studied by real-time quantitative PCR. Lthsp701 was strongly induced by high- and low-temperature stress, but Lthsc70 and Lthsp702 were not very sensitive to temperature changes. All three Lthsp70 s were expressed during insect development stages, but the expression patterns were quite different. The expression of Lthsc70 and Lthsp702 showed significant differences in expression during leafminer development; Lthsc70 was most highly expressed in female adults, whereas Lthsp702 was abundantly expressed in larvae and prepupae. Lthsp701 expression was not significantly different among leafminer stages. These results suggest that functional differentiation within the Lt HSP70 subfamily has occurred in response to thermal stress and insect development.