A novel integrated process of high cell-density culture combined with simultaneous saccharification and fermentation for ethanol production. (February 2019)

• Record Type: Journal Article
• Authors: Shi, Xuyang; Liu, Yan; Dai, Jinyu; Liu, Xintong; Dou, Sen; Teng, Lirong; Meng, Qingfan; Lu, Jiahui; Ren, Xiaodong; Wang, Runwei
• Is Part Of: Biomass and bioenergy. Volume 121(2019)
• Page Start: 115
• Page End: 121
• Publication Date: 2019-02
• Subjects: High cell-density culture -- Simultaneous saccharification and fermentation -- Ethanol -- Saccharomyces cerevisiae -- Corn stover; SSF simultaneous saccharification and fermentation -- HCDC high cell-density culture; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2018.12.020
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: A novel process integration occupying high cell-density culture (HCDC) and simultaneous saccharification and fermentation (SSF) in one bioreactor achieved an efficient ethanol production in this study. The effect of inoculum sizes of S. cerevisiae for ethanol production in SSF was investigated. High ethanol production was obtained with high inoculums size of cells from 4 to 30 kg﹒m −3 . The process integration including HCDC and SSF in one reactor was achieved. A production of 35.2 kg﹒m −3 cells was obtained with HCDC in aerobic conditions. Cellulase and pretreated corn stover were added into the fermentation broth in the same bioreactor, and the SSF procedure was initiated in anaerobic conditions. The highest ethanol titer of 17.08kg﹒m −3 corresponding to 101.50% theoretical yield was obtained, and the peak value time was 24 h with β-glucosidase addition in the HCDC-SSF system. The highest ethanol titer of 11.01 kg﹒m −3, corresponding 65.41% theoretical yield, was obtained at 72 h with regular SSF. Graphical abstract: Image 1 Highlights: The high cell density of yeast promoted the ethanol production in SSF. A novel process integration occupying high cell density culture and SSF shortened the overall process time and enhanced the ethanol production. The yeast culturing and SSF were combined in one bioreactor.