Combining orthogonal CRISPR and CRISPRi systems for genome engineering and metabolic pathway modulation in Escherichia coli. Issue 5 (8th February 2019)

• Record Type: Journal Article
• Authors: Sung, Li‐Yu; Wu, Meng‐Ying; Lin, Mei‐Wei; Hsu, Mu‐Nung; Truong, Vu Anh; Shen, Chih‐Che; Tu, Yi; Hwang, Kuen‐Yuan; Tu, An‐Pang; Chang, Yu‐Han; Hu, Yu‐Chen
• Is Part Of: Biotechnology and bioengineering. Volume 116:Issue 5(2019)
• Page Start: 1066
• Page End: 1079
• Publication Date: 2019-02-08
• Subjects: Cas9 ortholog -- CRISPR -- CRISPRi -- metabolic engineering -- SaCas9 -- St1Cas9; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.26915
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: CRISPR utilizing Cas9 from Streptococcus pyogenes (SpCas9) and CRISPR interference (CRISPRi) employing catalytically inactive SpCas9 (SpdCas9) have gained popularity for Escherichia coli engineering. To integrate the SpdCas9‐based CRISPRi module using CRISPR while avoiding mutual interference between SpCas9/SpdCas9 and their cognate single‐guide RNA (sgRNA), this study aimed at exploring an alternative Cas nuclease orthogonal to SpCas9. We compared several Cas9 variants from different microorganisms such as Staphylococcus aureus (SaCas9) and Streptococcus thermophilius CRISPR1 (St1Cas9) as well as Cas12a derived from Francisella novicida (FnCas12a). At the commonly used E. coli model genes LacZ, we found that SaCas9 and St1Cas9 induced DNA cleavage more effectively than FnCas12a. Both St1Cas9 and SaCas9 were orthogonal to SpCas9 and the induced DNA cleavage promoted the integration of heterologous DNA of up to 10 kb, at which size St1Cas9 was superior to SaCas9 in recombination frequency/accuracy. We harnessed the St1Cas9 system to integrate SpdCas9 and sgRNA arrays for constitutive knockdown of three genes, knock‐in pyc and knockout adhE, without compromising the CRISPRi knockdown efficiency. The combination of orthogonal CRISPR/CRISPRi for metabolic engineering enhanced succinate production while inhibiting byproduct formation and may pave a new avenue to E. coli engineering. Abstract : Orthogonal St1Cas9 and SpdCas9 can be combined for CRISPR/CRISPRi‐mediated metabolic engineering (gene integration, knockout, and suppression) to enhance succinate production in Escherichia coli .