Pathological effects of concurrent administration of aflatoxin B1 and fowl adenovirus-4 in broiler chicks. (August 2018)

• Record Type: Journal Article
• Authors: Naseem, Muhammad Noman; Saleemi, Muhammad Kashif; Khan, Ahrar; Khatoon, Aisha; Gul, Shafia Tehseen; Rizvi, Farzana; Ahmad, Ijaz; Fayyaz, Ahad
• Is Part Of: Microbial pathogenesis. Volume 121(2018)
• Page Start: 147
• Page End: 154
• Publication Date: 2018-08
• Subjects: Aflatoxins -- Adenovirus-4 -- Broiler Chicks -- Pathology -- Inclusion bodies -- Mortality; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.05.021
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The current study was designed to investigate pathological effects of fowl adenovirus in broilers exposed to aflatoxin B1 . Fowl Adenovirus-4 (FAdV-4) infection is remerging in all types of poultry birds in Pakistan. Poultry feed contamination with mycotoxin (aflatoxin) is another important global issue. A total of 125-day old broiler birds were divided into six equal groups. Group A served as control. B and C groups were administered with aflatoxin B1 (AFB1 ) 100 and 200 μg/kg feed. Group D was infected with FAdV-4, while groups E and F administered with both AFB1 (100 & 200 μg/kg) along with FAdV-4. These birds were monitored for clinical signs and mortality. Feed intake, body weight (BW), relative organ weights and gross & histopathological lesions were recorded. The highest mortality was observed in group F (FAdV-4 + AFB1 200 μg/kg feed) and the lowest mortality was observed in group B (AFB1 100 μg/kg feed). Body weights of all the groups were significantly ( p < 0.05) lower as compared with control group. Relative weight of liver and kidneys in groups E and F were significantly higher as compared with control. Grossly, liver was swollen, anemic with round margins in groups D, E and F. Kidneys were also swollen with whitish areas indicating dead tissue. Microscopically intranuclear inclusion bodies were observed in group D-F. The hepatic parenchyma was also indicating necrotic changes along with vacuolar degeneration. In renal parenchyma, acute tubular necrosis was observed in groups C, E and F. It was concluded that AFB1 intoxication lead to dose dependent changes in liver and kidneys. Severity of the changes was increased in interactive groups of AFB1 with FAdV-4. Therefore, feed should be regularly monitored for AFB1 levels and day old chicks for vertically transmitted FAdV-4 to prevent losses. Highlights: Concurrent Administration of Aflatoxin B1 and Fowl Adenovirus-4 in Broiler Chicks lead to various pathological lesions. Highest mortality observed in birds treated with high dose of AFB1 plus FAdV-4. Significantly lowered body weights and relative organ weight in groups treated with AFB1 plus FAdV-4 were recorded. Pathological lesions and intranuclear inclusion bodies were seen all groups treated with FAdV-4. Severity of the changes was increased in interactive groups of AFB1 with FAdV-4.