Distinctive Changes of L-Type Calcium Channels and Dopamine Receptors in the Dorsomedial and Dorsolateral Striatum after the Expression of Habitual Cocaine-Seeking Behavior in Rats. (1st February 2018)

• Record Type: Journal Article
• Authors: Shen, Fang; Jin, Shubo; Duan, Ying; Liang, Jing; Zhang, Meng; Jiang, Fengze; Sui, Nan
• Is Part Of: Neuroscience. Volume 370(2018)
• Page Start: 139
• Page End: 147
• Publication Date: 2018-02-01
• Subjects: CREB cAMP-response element binding protein -- D1R D1 receptors -- D2R D2 receptors -- DA dopamine -- DLS dorsolateral striatum -- DMS dorsomedial striatum -- DS dorsal striatum -- FI fixed interval -- LTCCs L-type calcium channels -- NAc nucleus accumbens -- RI random interval -- SA self-administration -- TBS Tris-buffered saline; Cav1.2 -- Cav1.3 -- dopamine receptors -- cocaine habitual seeking -- DMS -- DLS; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.07.049
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Forty percent of rats exhibited cocaine habitual seeking behavior after 10 days of changeable FI schedule. Cav 1.2 and D1R in the DLS in well-established cocaine habitual seeking rats demonstrated higher expression. Cav 1.3 and D2R in the DMS in well-established cocaine habitual seeking rats demonstrated lower expression. Abstract: Habitual drug-seeking behavior is essential in the transition from recreational drug use to compulsive drug use and is regulated by the dopamine (DA) system of the dorsal striatum (DS). However, a comparative study of the two subtypes of DA receptors, D1 receptors (D1R) and D2 receptors (D2R), which have opposite regulatory functions, in habitual drug-seeking behavior is absent. Moreover, the effects of L-type calcium channels (LTCCs) and the subtypes Cav 1.2 and Cav 1.3, which are downstream of D1R and D2R, respectively, on habitual drug-seeking behavior have yet to be revealed. Therefore, based on the establishment of habitual cocaine-seeking behavior with changeable fixed interval (FI) self-administration (SA) training in rats, we compared the distinctive changes in D1R vs. D2R and Cav 1.2 vs. Cav 1.3 in the expression of habitual cocaine-seeking behavior in the dorsomedial striatum (DMS) and dorsolateral striatum (DLS). Our results showed that approximately forty percent of the animals exhibited habitual behavior after cocaine SA training. In addition, the total and membrane Cav 1.2 and D1R in the DLS demonstrated higher expression, but the total and membrane Cav 1.3 and D2R in the DMS demonstrated lower expression in well-established cocaine habitual behavior animals compared with non-established habitual behavior animals. These results suggested that upregulation of D1R–Cav 1.2 signaling may enhance the function of the DLS and that inactivation of D2R–Cav 1.3 caused depressed activity in the DMS during expression of habitual cocaine-seeking behavior. The imbalanced function between the DMS and DLS, which causes a shift from the DMS to the DLS, may mediate habitual behaviors.