Antioxidant enzymes, hematology and histology of spleen in Nile tilapia fed supplemented diet with natural extracts challenged with Aeromonas hydrophila. Issue 79 (August 2018)

• Record Type: Journal Article
• Authors: Dotta, Geovana; de Andrade, Jaqueline Inês Alves; Garcia, Patrícia; Alves Jesus, Gabriel Fernandes; Mouriño, José Luiz Pedreira; Mattos, Jacó Joaquim; Dias Bainy, Afonso Celso; Martins, Maurício Laterça
• Is Part Of: Fish & shellfish immunology. Issue 79(2018)
• Page Start: 175
• Page End: 180
• Publication Date: 2018-08
• Subjects: Oreochromis -- Biochemistry -- Hematology -- Immunology -- Bacterium; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.05.024
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: This study investigated the effects of dietary supplementation with the extrats of propolis and Aloe barbadensis (aloe) on the antioxydant enzime activity, hematology and histology of the spleen of Nile tilapia challenged with Aeromonas hydrophila . Seventy two juvenile Nile tilapia were divided in four treatments and three replicates and fed extract mixture for 15 days: fish fed supplemented diet with 1% of the mixture of extracts of propolis and aloe (1:1) injected with phosphate-buffered saline (PBS); fish fed suplemented diet with 1% of the mixture of extracts of propolis and aloe (1:1) injected with the A. hydrophila, fish fed supplemented diet with the mixture of propolis extracts and aloe, injected with PBS and injected with A. hydrophila . The influence of the supplementation of propolis and Aloe extracts on the immunomodulation in tilapias was observed by the evaluation of the survival of the animals after challenge with A. hydrophila . Non-supplemented fish had a 44.5% survival rate and those supplemented with 1% of the mixture of extracts showed 55.6% survival 7 days after challenge. The supplemented animals also showed a significant increase in the number of lymphocytes in the evaluation of the blood parameters and, consequently, in the histopathological evaluation, presented greater presence of centers of melanomacrophages. In addition, the activity of the antioxidant enzymes glutathione reductase (GR) in the spleen presented a significant difference in fish supplemented with 1% of the extracts mixture, being superior in the animals injected with PBS when compared to those challenged with A. hydrophila . Highlights: Fish fed 1% extracts PBS injected showed higher activities of antioxidant enzymes glutathione reductase (GR) and catalase (CAT) in the spleen. The supplementation of propolis and Aloe extracts was able to subtly increase the survival of the animals after challenge with A. hydrophila. Nile tilapia fed supplemented diet with propolis and Aloe at 1% challenged with A. hydrophila increased the lymphocyte number.