Secretagogin is Expressed by Developing Neocortical GABAergic Neurons in Humans but not Mice and Increases Neurite Arbor Size and Complexity. (26th April 2017)

• Record Type: Journal Article
• Authors: Raju, Chandrasekhar S; Spatazza, Julien; Stanco, Amelia; Larimer, Phillip; Sorrells, Shawn F; Kelley, Kevin W; Nicholas, Cory R; Paredes, Mercedes F; Lui, Jan H; Hasenstaub, Andrea R; Kriegstein, Arnold R; Alvarez-Buylla, Arturo; Rubenstein, John L; Oldham, Michael C
• Is Part Of: Cerebral cortex. Volume 28:Number 6(2018)
• Page Start: 1946
• Page End: 1958
• Publication Date: 2017-04-26
• Subjects: gene coexpression -- human brain development -- interneurons -- neuronal maturation -- species differences; Cerebral cortex -- Periodicals; Brain -- Periodicals; 612.825
• DOI: 10.1093/cercor/bhx101
• Languages: English
• ISSNs: 1047-3211

Abstract

Abstract: The neocortex of primates, including humans, contains more abundant and diverse inhibitory neurons compared with rodents, but the molecular foundations of these observations are unknown. Through integrative gene coexpression analysis, we determined a consensus transcriptional profile of GABAergic neurons in mid-gestation human neocortex. By comparing this profile to genes expressed in GABAergic neurons purified from neonatal mouse neocortex, we identified conserved and distinct aspects of gene expression in these cells between the species. We show here that the calcium-binding protein secretagogin (SCGN) is robustly expressed by neocortical GABAergic neurons derived from caudal ganglionic eminences (CGE) and lateral ganglionic eminences during human but not mouse brain development. Through electrophysiological and morphometric analyses, we examined the effects of SCGN expression on GABAergic neuron function and form. Forced expression of SCGN in CGE-derived mouse GABAergic neurons significantly increased total neurite length and arbor complexity following transplantation into mouse neocortex, revealing a molecular pathway that contributes to morphological differences in these cells between rodents and primates.