Spatiotemporal control of gene expression boundaries using a feedforward loop. Issue 3 (23rd January 2020)

• Record Type: Journal Article
• Authors: Bandodkar, Prasad U.; Al Asafen, Hadel; Reeves, Gregory T.
• Other Names: Umulis David guestEditor.; Calve Sarah guestEditor.; Iber Dagmar guestEditor.
• Is Part Of: Developmental dynamics. Volume 249:Issue 3(2020)
• Page Start: 369
• Page End: 382
• Publication Date: 2020-01-23
• Subjects: dorsal morphogen -- Drosophila embryo -- feedforward loop -- gene expression model -- spatiotemporal dynamics -- transcription factor -- Twist -- Zelda; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.150
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: A feedforward loop (FFL) is commonly observed in several biological networks. The FFL network motif has been mostly studied with respect to variation of the input signal in time, with only a few studies of FFL activity in a spatially distributed system such as morphogen‐mediated tissue patterning. However, most morphogen gradients also evolve in time. Results: We studied the spatiotemporal behavior of a coherent FFL in two contexts: (a) a generic, oscillating morphogen gradient and (b) the dorsal‐ventral patterning of the early Drosophila embryo by a gradient of the NF‐κB homolog dorsal with its early target Twist. In both models, we found features in the dynamics of the intermediate node—phase difference and noise filtering—that were largely independent of the parameterization of the models, and thus were functions of the structure of the FFL itself. In the dorsal gradient model, we also found that proper target gene expression was not possible without including the effect of maternal pioneer factor Zelda. Conclusions: An FFL buffers fluctuation to changes in the morphogen signal ensuring stable gene expression boundaries. Key Findings: A phase difference between the morphogen signal and intermediate node results in a stable signal. Intermediate node acts as a noise filter. Proper target gene expression of the Dorsal system needs the inclusion of the effect of pioneer factor Zelda.