A pilot study to establish human T‐cell leukemia virus type 1 (HTLV‐1) carrier model using common marmoset (Callithrix jacchus). Issue 2 (12th January 2020)

• Record Type: Journal Article
• Authors: Hirose, Lisa; Hiramoto, Takafumi; Tian, Yamin; Kohara, Hiroshi; Kobayashi, Seiichiro; Nagai, Etsuko; Denda, Tamami; Tanaka, Yukihisa; Ota, Yasunori; Jiyuan, Liao; Miyamoto, Shohei; Miura, Yoshie; Hijikata, Yasuki; Soda, Yasushi; Inoue, Takashi; Okahara, Norio; Itoh, Toshio; Sasaki, Erika; Tojo, Arinobu; Uchimaru, Kaoru; Tani, Kenzaburo
• Is Part Of: Journal of medical primatology. Volume 49:Issue 2(2020)
• Page Start: 86
• Page End: 94
• Publication Date: 2020-01-12
• Subjects: ATLL -- common marmoset -- cyclosporine -- HTLV‐1 -- MT‐2 cells; Primates -- Periodicals; Primates -- Diseases -- Periodicals; Medicine, Experimental -- Periodicals; 616.02738
• DOI: 10.1111/jmp.12454
• Languages: English
• ISSNs: 0047-2565

Abstract

Abstract: Background: For the diagnosis and treatment of adult T‐cell leukemia/lymphoma (ATLL) caused by human T‐lymphotropic virus type 1 (HTLV‐1) are required therapeutic modalities urgently. Non‐human primate models for ATLL would provide a valuable information for clinical studies. We did a pilot study to establish an ATLL non‐human primate model using common marmosets ( Callithrix jacchus ). Methods: We inoculated HTLV‐1–producing MT‐2 cells into 9‐month‐old marmosets, either intraperitoneally or intravenously. We next administrated MT‐2 cells into 13‐month‐old marmosets under cyclosporine A (CsA) treatment to promote infection. HTLV‐1 infection was determined by measuring HTLV‐1 antibody titer in the common marmosets. Results: The HTLV‐1 antibody titer increased in the intraperitoneally inoculated marmoset with or without CsA treatment, and it kept over five 5 years though proviral copy number (proviral load, PVL) remained low throughout the study. Conclusion: We obtained HTLV‐1 asymptomatic carriers of common marmosets by inoculating MT‐2 cells.