Genetically engineered haemoglobin wrapped covalently with human serum albumins as an artificial O2 carrier. Issue 6 (16th December 2019)

• Record Type: Journal Article
• Authors: Funaki, Ryosuke; Okamoto, Wataru; Endo, Chihiro; Morita, Yoshitsugu; Kihira, Kiyohito; Komatsu, Teruyuki
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 6(2020)
• Page Start: 1139
• Page End: 1145
• Publication Date: 2019-12-16
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb02184a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Recombinant human haemoglobin expressed in Pichia yeast was wrapped covalently with recombinant human serum albumins, yielding a core–shell structured rHbA(X)–rHSA 3 cluster as an entirely synthetic O2 carrier used for a red blood cell substitute. Abstract : We describe the synthesis and O2 affinity of genetically engineered human adult haemoglobin (rHbA) wrapped covalently with recombinant human serum albumins (rHSAs) as an artificial O2 carrier used for a completely synthetic red blood cell (RBC) substitute. Wild-type rHbA [rHbA(wt)] expressed in yeast species Pichia pastoris shows an identical amino acid sequence and three-dimensional structure to those of native HbA. It is particularly interesting that two orientations of the prosthetic haem group in rHbA(wt) were aligned by gentle heating in the natural form. Covalent wrapping of rHbA(wt) with three rHSAs conferred a core–shell structured haemoglobin–albumin cluster: rHbA(wt)–rHSA 3 . Three variant clusters containing an rHbA mutant core were also created: Leu-β28 → Phe, Leu-β28 → Trp, and Leu-β28 → Tyr/His-β63 → Gln. Replacement of Leu-β28 with Trp decreased the distal space in the haem pocket, thereby yielding a cluster with moderately low O2 affinity which is nearly the same as that of human RBC.