Phytobacter palmae sp. nov., a novel endophytic, N2 fixing, plant growth promoting Gammaproteobacterium isolated from oil palm (Elaeis guineensis Jacq.). Issue 2 (12th December 2019)

• Record Type: Journal Article
• Authors: Madhaiyan, Munusamy; Saravanan, Venkatakrishnan Sivaraj; Blom, Jochen; Smits, Theo H. M.; Rezzonico, Fabio; Kim, Soo-Jin; Weon, Hang-Yeon; Kwon, Soon-Wo; Whitman, William B.; Ji, Lianghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 2(2020)
• Page Start: 841
• Page End: 848
• Publication Date: 2019-12-12
• Subjects: Enterobacteriaceae -- Enterobacterales -- Nitrogen fixation -- Endophytes; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003834
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel strain S29 T with high nitrogen fixing ability was isolated from surface-sterilized leaf tissues of oil palm ( Elaeis guineensis ) growing in Science Park II, Singapore. On the basis of 16S rRNA gene analysis and multilocus sequence typing with the rpoB, gyrB, infB and atpD genes, strain S29 T was a member of the genus Phytobacter, with Phytobacter ursingii ATCC 27989 T and Phytobacter diazotrophicus LS 8 T as its closest relatives. Unique biochemical features that differentiated strain S29 T from its closest relatives were the ability to utilize melibiose, α -cyclodextrin, glycogen, adonital, d -arabitol, m -inositol and xylitol. The major fatty acids were C14 : 0, C16 : 0, C17 : 0, C16 : 1 ω 5c and summed feature 2 containing C16 : 1 ω 7c and/or C16 : 1 ω 6c. The polar fatty acid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and aminolipids. The draft genome of strain S29 T comprised 5,  284, 330 bp with a G + C content of 52.6 %. The average nucleotide identity and digital DNA–DNA hybridization values between strain S29 T and the phylogenetically related Enterobacterales species were lower than 95 % and 70 %, respectively. Thus, the polyphasic evidences generated through the phenotypic, chemotaxonomic and genomic methods confirmed that strain S29 T represents a novel species of the genus Phytobacter, for which we propose the name Phytobacter palmae sp. nov. with the type strain of S29 T (=DSM 27342 T =KACC 17598 T ).