Identification of Quantitative Trait Loci for Oil Content in Soybean Seed. Issue 1 (1st January 2015)

• Record Type: Journal Article
• Authors: Wang, Jiao; Chen, Pengyin; Wang, Dechun; Shannon, Grover; Shi, Ainong; Zeng, Ailan; Orazaly, Moldir
• Is Part Of: Crop science. Volume 55:Issue 1(2015)
• Page Start: 23
• Page End: 34
• Publication Date: 2015-01-01
• Subjects: Crop science -- Periodicals; Cultures -- Périodiques; Cultures de plein champ -- Périodiques; Crop science; Nutzpflanzen; Zeitschrift; Pflanzenbau; Periodicals; 633
• DOI: 10.2135/cropsci2014.04.0280
• Languages: English
• ISSNs: 0011-183X

Abstract

ABSTRACT: Oil is one of the major chemical constituents that affect the quality of soybean [ Glycine max (L.) Merr.] products. Soybean seed with high oil content is a valuable source for cooking oil and biodiesel production. The objective of this study was to identify quantitative trait loci (QTL) for oil content to advance soybean breeding efforts. Two F2 –derived recombinant inbred line (RIL) populations, consisting of 242 and 214 individuals from the cross of R05–638 × R05–1415 (Population 1) and R05–4256 × V97–1346 (Population 2), respectively, were used for QTL mapping. The F2 plants from the mapping populations were genotyped by simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Seeds from F2:3, F2:4, and F2:5 lines were tested for oil content using near infrared transmittance technique based on 13% moisture. Six oil QTL regions located on chromosomes 5, 6, 14, 15, and 20 were identified. Among the QTL identified, a major oil QTL (14–40%) on chromosome 20 linked with eight SNP markers was confirmed across genetic populations, locations, and years; this QTL had negative effect on seed protein content. In Population 2, two novel QTL located on chromosomes 6 and 14 accounting for between 11 and 15% and 8 and 15% of the variation in seed oil content, respectively, were detected. These QTL with linked markers can be used for marker‐assisted selection for increased seed oil content in soybean breeding programs.