In‐Cell EPR: Progress towards Structural Studies Inside Cells. (26th September 2019)

• Record Type: Journal Article
• Authors: Bonucci, Alessio; Ouari, Olivier; Guigliarelli, Bruno; Belle, Valérie; Mileo, Elisabetta
• Is Part Of: Chembiochem. Volume 21:Number 4(2020)
• Page Start: 451
• Page End: 460
• Publication Date: 2019-09-26
• Subjects: EPR spectroscopy -- in-cell spectroscopy -- SDSL -- spin labels -- structural biology; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201900291
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Exploring the structure and dynamics of biomolecules in the context of their intracellular environment has become the ultimate challenge for structural biology. As the cellular environment is barely reproducible in vitro, investigation of biomolecules directly inside cells has attracted a growing interest. Among magnetic resonance approaches, site‐directed spin labeling (SDSL) coupled to electron paramagnetic resonance (EPR) spectroscopy provides competitive and advantageous features to capture protein structure and dynamics inside cells. To date, several in‐cell EPR approaches have been successfully applied to both bacterial and eukaryotic cells. In this review, the major advances of in‐cell EPR spectroscopy are summarized, as well as the challenges this approach still poses. Abstract : Looking into cells : Among magnetic resonances approaches, site‐directed spin labeling coupled to EPR spectroscopy exhibits competitive and advantageous features to analyze proteins inside cells. Several in‐cell EPR approaches have been successfully applied to bacterial and eukaryotic cells. Here, the major advances of in‐cell EPR and the challenges of this approach are summarized.