A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate chromophore‐binding pattern. Issue 4 (24th November 2019)

• Record Type: Journal Article
• Authors: Yan, Hui; Zhu, Kongfu; Teng, Maikun; Li, Xu
• Is Part Of: FEBS letters. Volume 594:Issue 4(2020)
• Page Start: 740
• Page End: 750
• Publication Date: 2019-11-24
• Subjects: Ap‐phr -- CRY‐DASH -- crystal structure -- enzyme activity -- MTHF; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13657
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Cyclobutane pyrimidine dimers (CPD), as a common DNA damage caused by UV radiation, often lead to skin cancer. Here, we identified a photolyase from the alga Arthrospira platensis (designated as Ap‐ phr), which has been regarded as a safe organism for humans for centuries, that can efficiently repair CPD lesions in ssDNA and dsDNA in vitro . The 1.6 Å resolution crystal structure of Ap‐ phr revealed that it possesses a unique methenyltetrahydrofolate chromophore‐binding pattern with high energy transfer efficiency. Our study of Ap‐ phr highlights its potential use in cosmetic, industrial and aesthetic medicine applications. Abstract :