Extraction of 3-acetyl-2, 5-dimethylthiophene and purification the fast food noodle samples using a novel acid–base-induced cloud point extraction and magnetic solid-phase extraction prior to HPLC. Issue 6 (12th April 2020) - Zhao, Jiao
ABSTRACT: A novel method based on acid–base-induced cloud point extraction (AB-CPE) and magnetic solid-phase extraction (MSPE) coupled with high performance liquid chromatography (HPLC) for extraction and purification of 3-acetyl-2, 5-dimethylthiophene (ADP) in the fast food noodle samples was established for the first time. In MSPE-AB-CPE, Fe3 O4 @SiO2 nanoparticles are used to purify samples, medium-chain saturated fatty acids ( n -octanoic acid), and nonionic surfactant (TMN-6) were investigated as a pH-dependent phase based on acid–base reaction caused solely by adjusting pH. The calibration curves demonstrated linearity in the range 0.5–100 µg mL −1 ( R 2 = 0.9969), with relative recoveries (95.7–101.2%) and relative standard deviations (2.6–4.8%, n = 5).