Novel dammarane-type saponins from Gynostemma pentaphyllum and their neuroprotective effect. Issue 5 (3rd March 2020)

• Record Type: Journal Article
• Authors: Xing, Shao-Fang; Lin, Man; Wang, Yu-Rong; Chang, Tuo; Cui, Wei-Ye; Piao, Xiang-Lan
• Is Part Of: Natural product research. Volume 34:Issue 5(2020)
• Page Start: 651
• Page End: 658
• Publication Date: 2020-03-03
• Subjects: Gynostemma pentaphyllum -- dammarane-type saponin -- gypenoside -- neuroprotective effect -- SH-SY5Y; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1495638
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Three novel dammarane-type saponins, 2 α, 3 β, 12 β, 20( S ), 24( S )-pentahydroxydammar-25-ene-3- O - β -D-glucopyranosyl-(1→2)- β -D-glucopyranosyl-20- O - β -D-glucopyranoside (1, namely gypenoside J1), 2 α, 3 β, 12 β, 20( S ), 25-pentahydroxydammar-23-ene-3- O - β -D-glucopyranosyl-(1→2)- β -D-glucopyranosyl-20- O - β -D-glucopyranoside (2, namely gypenoside J2) and 2 α, 3 β, 12 β, 20(S)-tetrahydroxydammar-25-en-24-one-3- O - β -D-glucopyranosyl-(1→2)- β -D-glucopyranosyl-20- O - β -D-xylopyranosyl-(1→6)- β -D-glucopyranoside (3, namely gypenoside J3) along with one known gypenoside (gypenoside LVII) were isolated from the aerial parts of G. pentaphyllum using various chromatographic methods. Their structures were elucidated on the basis of IR, 1D- ( 1 H and 13 C), 2D-NMR spectroscopy (HSQC, HMBC and COSY), and mass spectrometry (ESI-MS/MS). Their activity was tested using CCK-8 assay. These four compounds showed little anti-cancer activity with IC50 values more than 100 μM against four types of human cancer lines. The effects of them against H2 O2 -induced oxidative stress in human neuroblastoma SH-SY5Y cells were evaluated and they all showed potential neuroprotective effects with 3.64–18.16% higher cell viability than the H2 O2 -induced model group. Abstract :