Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli. Issue 17 (4th February 2020)

• Record Type: Journal Article
• Authors: Savage, Jonathan C.; Davare, Monika A.; Shinde, Ujwal
• Is Part Of: Chemical communications. Volume 56:Issue 17(2020)
• Page Start: 2634
• Page End: 2637
• Publication Date: 2020-02-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc09375c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Spectroscopic and kinetic investigations of tripartite complex formation reveal differences between Broccoli and Spinach aptamers, highlighting how subtle sequence variations impart functional consequences of G-Quadruplex–cation interactions in RNA. Abstract : Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex–cation interactions in RNA.