Electrochemical biosensor for detection of 17β-estradiol using semi-conducting polymer and horseradish peroxidase. Issue 15 (3rd March 2020)

• Record Type: Journal Article
• Authors: Spychalska, Kamila; Zając, Dorota; Cabaj, Joanna
• Is Part Of: RSC advances. Volume 10:Issue 15(2020)
• Page Start: 9079
• Page End: 9087
• Publication Date: 2020-03-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c9ra09902f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A convenient electrochemical sensing pathway for 17β-estradiol detection was investigated. Abstract : A convenient electrochemical sensing pathway for 17β-estradiol detection was investigated. The system is based on a conducting polymer and horseradish peroxidase (HRP) modified platinum (Pt) electrode. The miniature estradiol biosensor was developed and constructed through the immobilization of HRP in an electroactive surface of the electrode covered with electroconducting polymer – poly(4, 7-bis(5-(3, 4-ethylenedioxythiophene)thiophen-2-yl)benzothiadiazole). The detection strategy is based on the fact that 17β-estradiol (E2) and pyrocatechol (H2 Q) are co-substrates for the HRP enzyme. HRP, which does not react with E2, in the presence of H2 O2 catalyses the oxidation of H2 Q to o -benzoquinone (Q). With the optimized conditions, such constructed biosensing system demonstrated a convenient level of sensitivity, selectivity in a broad linear range – 0.1 to 200 μM with a detection limit of 105 nM. Furthermore, the method was successfully applied for hormone detection in the presence of potential interfering compounds (ascorbic acid, estriol, estrone, uric acid and cholesterol).