Structural characterization, antioxidant and immunomodulatory activities of a neutral polysaccharide from Cordyceps militaris cultivated on hull-less barley. (1st May 2020)

• Record Type: Journal Article
• Authors: Zhang, Ying; Zeng, Yan; Cui, Yongsheng; Liu, Hanmin; Dong, Caixia; Sun, Yuanxia
• Is Part Of: Carbohydrate polymers. Volume 235(2020)
• Publication Date: 2020-05-01
• Subjects: Polysaccharide -- Cordyceps militaris -- Hull-less barley -- Antioxidant activity -- Immunomodulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.115969
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A polysaccharide was isolated from Cordyceps militaris cultivated on hull-less barley. The heteropolysaccharide had a backbone of (1→2)- α -D-Man p. The heteropolysaccharide was mainly branched at O -6 position by (1 → 2)- β -D-Gal f. The heteropolysaccharide had antioxidant and immunoregulation potentials. Abstract: A water-soluble neutral polysaccharide (SDQCP-1) was isolated from fruit bodies of Cordyceps militaris cultivated on hull-less barley. SDQCP-1 was composed of mannose, glucose and galactose in the mole ratio of 13.3:1.0:9.7, with an average molecular weight of 19.3 kDa. Based on results from methylation analysis, GC–MS and NMR, SDQCP-1 was elucidated to be a glucogalactomannan with a backbone composed of (1→2)- α -D-Man p (48.4 %) and (1 → 4)- β -D-Glc p (1.2 %) residues. Its side chains were branched at O -6 position of (1→2)- α -D-Man p mainly by (1 → 2)- β -D-Gal f or (1 → 6)- α -D-Man p residues which were terminated mainly with α -D-Gal f, α -D-Gal p residues. Besides exhibiting a good antioxidant capacity with an ORACFL value of 24.7 mmol Trolox/g and a TEAC value of 202.4 μmol Trolox/g, SDQCP-1 also could stimulate macrophages to release NO, TNF-α, IL-6, and IL-10, and mainly induced M1 polarization of macrophages. The findings indicated that SDQCP-1, from C. militaris cultivated on hull-less barley, could be used as potential natural antioxidant and immunomodulator in functional foods or medicine.