A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer. (21st February 2020)

• Record Type: Journal Article
• Authors: Liu, Yabin; Li, Binghui; Wang, Lili; Kong, Dexian
• Other Names: Zeigler-Johnson Charnita M. Academic Editor.
• Is Part Of: BioMed research international. Volume 2020(2020)
• Publication Date: 2020-02-21
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2020/2091781
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Objective . To investigate whether the polymorphisms of interleukin-12B ( IL-12B ) were associated with the risk of developing colorectal cancer (CRC). Patients and Methods . Genotypes of rs17860508 and rs3212227 were determined by polymerase chain reaction with a direct sequencing method in 329 CRC patients and 342 matched healthy control subjects. The expression of IL-12B mRNA was determined by RT-qPCR in 50 pairs of CRC tissues and their adjacent normal tissues. Results . Compared with TTAGAG/TTAGAG genotype of rs17860508, the GC/GC and TTAGAG/GC genotypes may significantly increase the risk of CRC (OR = 1.81, 95% CI = 1.18–2.78; OR = 1.46, 95% CI = 1.01–2.12, respectively). Furthermore, the mRNA levels of IL-12B were significantly higher in the CRC tissues from patients with the rs17860508 GC/GC genotype than those with the TTAGAG/GC (P = 0.009 ) and TTAGAG/TTAGAG (P = 0.001 ) genotypes. Conclusion . These data suggested that the rs17860508 GC/GC genotype might upregulate IL-12B expression at the transcriptional level and thus increase the risk of CRC.