"Off/on" fluorescence imaging-guided cancer diagnosis and multi-modal therapy. (21st January 2020)

• Record Type: Journal Article
• Authors: Wang, Changrong; Dong, Yanliang; Shi, Xiaoguang; Guo, Jinxuan; Zhang, Jianhua; Deng, Liandong; Lin, Zhiqiang; Huang, Pingsheng; Shi, Yongli; Wang, Weiwei; Dong, Anjie
• Is Part Of: Biomaterials science. Volume 8:Number 5(2020)
• Page Start: 1442
• Page End: 1454
• Publication Date: 2020-01-21
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c9bm01854a
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : An efficient theranostic nanoplatform responding to tumour microenvironments with characters of simple and flexible combinations owns great potential in cancer diagnosis and therapy. Abstract : An efficient theranostic nanoplatform responding to tumour microenvironments with characters of simple and flexible combinations owns great potential in cancer diagnosis and therapy. Herein, a series of triblock copolymers, mPEG- b -PDPA- b -P( n BMA-r-cystamine) (EPB), were synthesized and among them, the structure of EPB-3 was optimized for both fluorescence imaging-guided cancer diagnosis and multi-modal therapy with good biocompatibility. (1) The self-assembled nanoparticles of EPB-3-ICG1 obtained by conjugating one ICG on EPB-3 via S–S bonds effectively performed reduction-sensitive OFF/ON fluorescence signal transition, thus inducing tumour cell-specific amplified fluorescence imaging in vitro and in vivo . (2) By entrapping Au nanorods into the co-assembled NPs of EPB-3 and EPB-3-ICG1, EPB-3-ICG1 @Au NPs could synchronously induce strong tumour fluorescence imaging and high local photothermal effect, indicating the potential of imagine-guided photothermal therapy. (3) EPB-3 NPs could efficiently co-load paclitaxel (PTX) and ICG to form stable EPB-3@PTX@ICG NPs, which provided long periods of intracellular pH-sensitive sustainable drug release and highly enhanced apoptosis of 4T1 cells in vitro by the chemo-photothermal effect. Excitingly, a single intravenous injection of EPB-3@PTX@ICG NPs followed by a one-time local near-infrared light (NIR, 808 nm) irradiation treatment for 10 min could lead to significant inhibition of tumour growth, avoiding tumor metastasis and extending the survival of mice. All the above-mentioned results suggest that EPB-3 provides a nanoplatform with the characters of simple structure, convenience of use and flexible combination, holding potential for multi-modal diagnosis and therapy.