Nitrilotriacetic acid-end-functionalized polycaprolactone as a template for polymer–protein nanocarriers. Issue 9 (17th January 2020)

• Record Type: Journal Article
• Authors: Jose, Leeja; Hwang, Aran; Lee, Chaeyeon; Shim, KyuHwan; Song, Jae Kwang; An, Seong Soo A.; Paik, Hyun-jong
• Is Part Of: Polymer chemistry. Volume 11:Issue 9(2020)
• Page Start: 1580
• Page End: 1588
• Publication Date: 2020-01-17
• Subjects: Polymers -- Periodicals; Macromolecules -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1039/c9py01663e
• Languages: English
• ISSNs: 1759-9954

Abstract

Abstract : Doxorubicin loaded Nickel-complexed nitrilotriacetic acid-end-functionalized polycaprolactone based biocompatible polymer–protein hybrid nanocarriers were developed in a one-pot process. Abstract : Diverse polymer–protein nanostructures were introduced as promising platforms for biomedical applications based on their unification of biocompatibility, the bespoke functionalities of proteins, and the structural integrity of polymers. Previously, the polymer-templated protein nanoball (PTPNB) system with Ni 2+ -nitrilotriacetic acid-end-functionalized polystyrene (Ni 2+ -NTA-PS) and His6 -GFP was reported. These synthesized nanostructures maintained a high protein activity and protein orientation through a soft and strong specific Ni 2+ -NTA/histidine synergy between the protein and the polymer. However, the toxicity of polystyrene (PS) was a major limitation of the PTPNB system for biomedical applications. Herein, biocompatible polymer–protein nanocarriers were developed by synthesizing nickel-complexed nitrilotriacetic acid-end-functionalized polycaprolactone (Ni 2+ -NTA-PCL). The potentiality of the current system was the preparation of polymer–protein hybrid nanocarriers with the loaded doxorubicin (DOX) in a one-pot process. Finally, the capability of DOX-loaded GFP/PCL as a therapeutic vehicle was verified by the cellular internalization and cytotoxicity test with neuroblastoma SH-SY5Y cells.