Protein fraction from Artocarpus altilis pulp exhibits antioxidant properties and reverses anxiety behavior in adult zebrafish via the serotoninergic system. (March 2020)

• Record Type: Journal Article
• Authors: Gonçalves, Nidyedja Goyanna Gomes; de Araújo, José Ismael Feitosa; Magalhães, Francisco Ernani Alves; Mendes, Francisco Rogênio Silva; Lobo, Marina Duarte Pinto; Moreira, Ana Cristina de Oliveira Monteiro; Moreira, Renato de Azevedo
• Is Part Of: Journal of functional foods. Volume 66(2020)
• Publication Date: 2020-03
• Subjects: Anxiolytic activity -- Breadfruit pulp -- Light/dark test -- Bioactive protein -- Nutraceutical; 5-HT, serotonin 5-hydroxytryptamine -- 5-HTR serotonin receptor -- ACN acetonitrile -- AOAC Association of Official Agricultural Chemists -- ATCC American Type Culture Collection -- BDZ benzodiazepines -- CTL control -- DMEM Dulbecco's modified Eagle's medium -- DT-PFBp denatured protein rich fraction of breadfruit pulp -- DTT dithiothreitol -- DZP diazepam -- Flx fluoxetine -- FTIR Fourier transform infrared spectroscopy -- GABA gamma-aminobutyric acid -- HEK293 human embryonic kidney 293 cells -- HPLC high-performance liquid chromatography -- IAA iodoacetamide -- IR infrared -- KBr potassium bromide -- LC-MS/MS liquid chromatography-tandem mass spectrometry -- LDT light/dark test -- MS mass spectrometry -- OFT open field test -- PFBp protein rich fraction of breadfruit pulp -- PITC phenylisothiocyanate -- PLZ permanence on the light zone -- RBC red blood cell -- RFU relative fluorescence unit -- RP-HPLC reverse-phase liquid chromatography -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- S.E.M. standard error of the mean -- TFA trifluoroacetic acid -- TLC thin layer chromatography; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103772
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: PFBp is a protein isolate from breadfruit with nutraceutical potential. PFBp contains high levels of essential amino acids. PFBp has metal-chelating activity. PFBp induces anxiolysis in zebrafish anxiety models via the serotoninergic system. Abstract: Breadfruit ( Artocarpus altilis ) is a staple food in tropical regions and has several uses. The aim of this study was to establish the nutraceutical potential of an enriched protein fraction of breadfruit pulp (PFBp). PFBp was characterized by Fourier transform infrared spectroscopy, electrophoresis, zymography, thin-layer chromatography, and mass spectrometry. PFBp toxicity was assessed in vitro and in vivo. Its pharmacological effects were determined using zebrafish anxiety models, open field test, and light/dark test. PFBp is rich in essential amino acids (49.59 g/100 g), predominantly lysine and leucine. It contains proteases, including cysteine proteases and three major proteins (20–30 kDa). PFBp was not cytotoxic and showed ion-chelating antioxidant activity. It induced anxiolysis in zebrafish anxiety models. This effect was mediated by the serotoninergic system, mainly, 5-HTR1A, 5-HTR2C, and 5-HTR3 . PFBp is a natural nutraceutical product. As a food supplement, PFBp could improve and sustain mental health.