Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro. (March 2020)

• Record Type: Journal Article
• Authors: Gajic, Dragica; Saksida, Tamara; Koprivica, Ivan; Vujicic, Milica; Despotovic, Sanja; Savikin, Katarina; Jankovic, Teodora; Stojanovic, Ivana
• Is Part Of: Journal of functional foods. Volume 66(2020)
• Publication Date: 2020-03
• Subjects: Aronia melanocarpa -- Type 1 diabetes -- Dendritic cells -- Macrophages -- T helper 1 cells; BSA bovine serum albumin -- ConA concanavalin A -- DC dendritic cell -- EDTA ethylenediaminetetraacetic acid -- FCS fetal calf serum -- GALT gut-associated lymphoid tissue -- IEL intraepithelial lymphocytes -- IFN-γ interferon-γ -- IL-1β interleukin-1β -- IL-17 interleukin-17 -- IL-6 interleukin-6 -- LPS lipopolysaccharide -- NO nitric oxide -- PC peritoneal cell -- PP Peyer's patches -- STZ streptozotocin -- T1D type 1 diabetes -- Th T helper cell -- Th1 type 1 T helper cell -- Th17 type 17 T helper cell -- TNF tumor necrosis factor -- Treg T regulatory cell; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103836
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Chokeberry extract modulates gut-associated immune response in healthy C57BL/6 mice. It aggravates hyperglycemia during type 1 diabetes development in C57BL/6 mice. It stimulates dendritic cell and macrophage pro-inflammatory functions in vitro. Differentiation of T helper 1 cells is stimulated upon extract application in vitro. Abstract: Chokeberry ( Aronia melanocarpa ) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro . When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1β production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-γ-producing T cells in vitro . The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity.