Enhancement of productivity of Chlorella pyrenoidosa lipids for biodiesel using co-culture with ammonia-oxidizing bacteria in municipal wastewater. (May 2020)

• Record Type: Journal Article
• Authors: Zhou, Xu; Jin, Wenbiao; Wang, Qing; Guo, Shida; Tu, Renjie; Han, Song-fang; Chen, Chuan; Xie, Guojun; Qu, Fanqi; Wang, Qilin
• Is Part Of: Renewable energy. Volume 151(2020)
• Page Start: 598
• Page End: 603
• Publication Date: 2020-05
• Subjects: Ammonia-degrading bacteria -- Biodiesel -- Chlorella pyrenoidosa -- Lipid -- Microalgae; COD Chemical oxygen demand -- NH3–N Ammonia-nitrogen -- OD Optical density -- SS Suspended solid -- TP Total phosphorus -- TN Total nitrogen -- Saturated fatty acids SFAs -- Monounsaturated fatty acids MUFAs -- Polyunsaturated fatty acids PUFAs; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2019.11.063
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: As one of the most promising renewable energy, microalgal biodiesel has been widely studied worldwide. However, the low-efficiency of conventional microalgae cultivation procedures restrict the development of microalgae biodiesel production. Microalgal-bacterial symbiosis could both enhance the growth of algal-bacterial culture and promote the removal and conversion of wastewater nutrients. In this study, three strains of high-efficient heterotrophic ammonia-oxidizing bacteria JN1, FN3, and FN5 were screened from municipal wastewater treatment system with over 80% degradation rates of 50 mg/L ammonia-nitrogen (NH3 –N) in 24 h. Among them, FN5, belonging to Kluyvera sp., had the optimum effect on enhancing growth of oil-rich microalga Chlorella pyrenoidosa . In stationary phase, the biomass and lipid content of Chlorella pyrenoidosa was14.8% and 13.6% higher than the blank control tests without FN5. In contrast, JN1 and FN3 failed to enhance the growth of Chlorella pyrenoidosa . After the cultivation of Chlorella pyrenoidosa -FN5 consortia in municipal wastewater, the degradation rate of NH3 –N was up to 91% while the content of microalgae biomass and lipid attained 0.35 g/L and 39.0%. The Saturated fatty acids (SFAs), Monounsaturated fatty acids (MUFAs), and Polyunsaturated fatty acids (PUFAs) were 43.9, 37.1 and 19.0%, respectively, which had the potential for biodiesel production after pretreatment. Highlights: High-efficient ammonia-oxidizing strain FN5 was identified and enriched. FN5 enhanced biomass concentration and lipid content of microalga Chlorella pyrenoidosa. Chlorella pyrenoidosa- FN5 culture removed NH3–N while accumulating higher microalgal lipid amounts.