Bufadienolides from the whole plants of Helleborus foetidus and their cytotoxicity. (April 2020)

• Record Type: Journal Article
• Authors: Iguchi, Tomoki; Yokosuka, Akihito; Kawahata, Riko; Andou, Madoka; Mimaki, Yoshihiro
• Is Part Of: Phytochemistry. Volume 172(2020)
• Publication Date: 2020-04
• Subjects: Helleborus foetidus -- Ranunculaceae -- Bufadienolides -- Bufadienolide glucosides -- Cytotoxicity -- Apoptosis -- HL-60 cells -- A549 cells; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2020.112277
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Two undescribed bufadienolide glucosides and four undescribed bufadienolides were isolated from the whole plants of Helleborus foetidus (Ranunculaceae). Their structures were determined by extensive spectroscopic analysis and the results of hydrolytic cleavage. The isolated compounds exhibited cytotoxic activities against HL-60 and A549 cells with IC50 values ranging from 0.019 to 3.0 μM. The isolated compounds also showed the Na + /K + ATPase inhibitory activity. The undescribed compound 16β-formyloxy-10, 14-dihydroxy-5β-[(β-d -glucopyranosyl)oxy]-19-norbufa-3, 20, 22-trienolide induced apoptosis in HL-60 cells through a mitochondria-dependent apoptotic pathway. Graphical abstract: Two undescribed bufadienolide glucosides and four undescribed bufadienolides were isolated from the whole plants of Helleborus foetidus (Ranunculaceae). The isolated compounds exhibited cytotoxic activities against HL-60 human leukemia cells and A549 human lung adenocarcinoma cells. Image 1 Highlights: Six undescribed bufadienolide derivatives were isolated from Helleborous foetidus. Structures were determined by spectroscopic analysis and the results of hydrolysis. Six compounds showed cytotoxicity against both HL-60 and A549 cells. The undescribed bufadienolide glycoside induced apoptosis in HL-60 cells.