Effect of dietary tryptophan supplementation on growth performance, immune response and anti‐oxidant status of broiler chickens from 7 to 21 days. Issue 1 (5th November 2019)

• Record Type: Journal Article
• Authors: Mund, Muhammad D.; Riaz, Muhammad; Mirza, Muhammad A.; Rahman, Zia‐ur; Mahmood, Tahir; Ahmad, Fawad; Ammar, Ahmad
• Is Part Of: Veterinary medicine and science. Volume 6:Issue 1(2020)
• Page Start: 48
• Page End: 53
• Publication Date: 2019-11-05
• Subjects: broiler -- growth performance -- immune response -- oxidant and anti‐oxidant status -- tryptophan; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.195
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Background: This study was conducted to investigate the optimum dietary level of tryptophan (Trp) supplementation at which broiler chickens have better growth with efficient immune system and anti‐oxidant status. Method: One hundred and twenty ( n = 120) 1‐day‐old broiler chicks were fed a common commercial diet from days 1 to 7. On day 7, the chicks were randomly divided in three treatment groups, that is, Trp 0.2 [national research council (NRC) recommended level of tryptophan], Trp 0.3 (tryptophan supplemented at 0.3%) and Trp 0.5 (tryptophan supplemented at 0.5%). All the experimental diets were iso‐caloric (ME; 3, 000 kcal/kg) and iso‐proteic (CP; 18.5%). Weekly data on feed intake and body weight gain (BWG) were recorded to calculate feed conversion ratio (FCR). On day 19, avian tuberculin was injected to note the cellular immunity. On day 21, two birds per replicate were killed to determine carcass and visceral organ weights. Blood serum samples were collected for analysis of humoral immune response against sheep red blood cells, total oxidant and anti‐oxidants by spectrophotometric method. Results: Feed intake, carcass and visceral organ weights remained unaffected by dietary treatments while BWG and FCR tended to improve ( p < .05) in broiler chicks fed the Trp 0.3 and the Trp 0.5 diets. Total oxidant status was also improved ( p < .05) in broiler chicks fed the Trp 0.5 diet. Likewise, broiler chicks fed the Trp 0.3 and the Trp 0.5 diets tended to have better ( p < .05) total anti‐oxidant status, catalase, glutathione peroxidase, glutathione reductase and arylesterase (ARE). The overall antibodies response and IgG improved ( p < .05) by the Trp 0.3 and Trp 0.5 diets compared to control. However, IgM level remained similar across the treatment. The cellular immunity against avian tuberculin improved at 24 hr post‐injection but its effect disappeared at 48 hr. Conclusion: The results of present study revealed that Trp above the NRC recommended level may give better growth, immune response and anti‐oxidant status in broiler chickens. Abstract : Broiler is a fast growing bird and facing different kind of problem. To address these problems, nutritional manipulation is the best strategy to overcome the difficulties in broiler rearing. Therefore, this study was planned to highlight the role of tryptophan in broiler nutrition to combat the disease condition and improve growth performance.