Disposition and measured toxicity of zinc oxide nanoparticles and zinc ions against keratinocytes in cell culture and viable human epidermis. Issue 2 (7th February 2020)

• Record Type: Journal Article
• Authors: Holmes, Amy M.; Mackenzie, Lorraine; Roberts, Michael S.
• Is Part Of: Nanotoxicology. Volume 14:Issue 2(2020)
• Page Start: 263
• Page End: 274
• Publication Date: 2020-02-07
• Subjects: Zinc oxide nanoparticles -- zinc ion species -- sunscreens -- toxicity; Toxicology -- Periodicals; 615.9
• DOI: 10.1080/17435390.2019.1692382
• Languages: English
• ISSNs: 1743-5390

Abstract

Abstract: Suspensions of the UV filter, zinc oxide nanoparticles (ZnO NP), are widely used in sunscreen products. This paper compared the relative disposition and local cytotoxicity of ZnO NP, and zinc ions formed on its dissolution, against keratinocyte cultures and in the human epidermis ( ex vivo ) after application of suspensions of ZnO NP. HaCaT keratinocyte cytotoxicities were found to be related to labile intra-cellular zinc but also total zinc and extra-cellular concentrations in cell culture media and to a degree ameliorated by the presence of a zinc chelating agent. Secondly, the zinc species were then dosed onto exposed ex vivo viable human epidermis and it was found that an increase in labile zinc level correlated with a shift in the metabolic state of the viable epidermis. This study highlights that excised viable skin acts as a more relevant model for determining cutaneous toxicity over keratinocyte monolayers in vitro .