A FRET-based ratiometric fluorescent probe to detect cysteine metabolism in mitochondria. Issue 7 (6th February 2020)

• Record Type: Journal Article
• Authors: Han, Xiaojing; Zhai, Zhiyao; Yang, Xiaopeng; Zhang, Di; Tang, Jun; Zhu, Jianming; Zhu, Xiaofei; Ye, Yong
• Is Part Of: Organic & biomolecular chemistry. Volume 18:Issue 7(2020)
• Page Start: 1487
• Page End: 1492
• Publication Date: 2020-02-06
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d0ob00002g
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A FRET fluorescent probe was reported for detecting the Cys metabolism in mitochondria. Abstract : As an important biothiol in living cells, cysteine is closely related to oxidative damage in living organisms. Sulfite from cysteine metabolism in living cells plays a crucial role in maintaining homeostasis in an organism, and the unbalance of sulfite in vivo would lead to multiple diseases. Thus the development of a new fluorescent probe for cysteine metabolism is needed urgently in mitochondria which are the main place of cysteine metabolism. Herein we construct a novel targeting mitochondria fluorescent probe CP-K based on the FRET mechanism to visualize sulfite in living MCF-7 cells. Probe CP-K displays a large Stokes shift of 150 nm, a low detection limit (26.3 nM) and "naked eye" detection after the addition of HSO3 − . Importantly, it is appropriate for imaging the endogenous sulfite from cysteine metabolism in living cells.