A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell. Issue 7 (23rd January 2020)

• Record Type: Journal Article
• Authors: Wang, Fu-Ting; Wang, Yi-Han; Xu, Jing; Huang, Ke-Jing
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 7(2020)
• Page Start: 1389
• Page End: 1395
• Publication Date: 2020-01-23
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb02574j
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification. Abstract : A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification. A nitrogen doped ultra-thin carbon shell/gold nanoparticle (N-UHCS/AuNPs) composite was prepared and applied as an electrode supporting substrate to improve the enzyme load. The biocathode of the self-powered sensor is constructed through the step-by-step modification of N-UHCS/AuNPs and bilirubin oxidase (BOD) on carbon paper (CP). To fabricate the bioanode, SiO2 nanospheres@AuNPs–aptamer (SiO2 @AuNPs–ssDNA) bioconjugates were prepared and modified on CP. When there is a target protein, the aptamer recognizes it and causes the SiO2@AuNPs–ssDNA bioconjugate to fall off the bioanode, resulting in a significant increase in the open circuit voltage ( E OCV ) of the sensing device. Under optimal conditions, the developed biosensor shows a wide linear range of 0.1–2000 ng mL −1 with a low detection limit of 21.5 pg mL −1 (S/N = 3). This work shows an effective assay for the sensitive detection of biomolecules by coupling EBFCs, DNA bioconjugates and the biosensing characteristics of smart nanostructures.