Risk assessment and biophysiochemical responses of spinach to foliar application of lead oxide nanoparticles: A multivariate analysis. (April 2020)

• Record Type: Journal Article
• Authors: Natasha, ; Shahid, Muhammad; Farooq, Abu Bakr Umer; Rabbani, Faiz; Khalid, Sana; Dumat, Camille
• Is Part Of: Chemosphere. Volume 245(2020)
• Publication Date: 2020-04
• Subjects: lead oxide nanoparticles -- Foliar application -- Biophysiochemical changes -- Risk assessment; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.125605
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Despite extensive research progress in the recent past, the data regarding foliar uptake of heavy metals, associated biophysiochemical changes inside plants and possible health hazards are limited. This study determined the effect of foliar application of lead oxide nanoparticles (PbO-NPs) on lead (Pb) accumulation, physiological and biochemical changes inside spinach plants and associated health risks. A green method was used to prepare PbO-NPs using coconut water. Scanning electron microscopy (SEM) showed the preparation of smooth, unwrinkled, granular and spherical PbO-NPs. Spinach leaves were exposed via foliar application to three concentrations of PbO-NPs (0, 10 and 50 mg/plant). Foliar PbO-NPs application resulted in a significant accumulation of Pb in leaves (42.25 μg g −1 ), with limited translocation towards root tissues (4.46 μg g −1 ). This revealed that spinach can accumulate considerable amount of Pb via foliar uptake. Lead accumulation inside spinach caused a significant decrease in pigment contents (38%) and dry weight (67%). After foliar uptake, Pb caused several-fold increase in the activities of catalase and peroxidase. However, foliar PbO-NPs did not induce significant changes in H2 O2 production, lipid peroxidation and superoxide dismutase activity. Application of PbO-NPs (50 mg/plant) showed possible health risks (non-carcinogenic) due to ingesting Pb-contaminated leaves of spinach. It is proposed that atmospheric contamination and foliar deposition of metal-PM can seriously affect vegetable growth and can provoke health issues due to ingestion of metal-enriched vegetables. Therefore, atmospheric levels of heavy metals need to be monitored on a regular basis to avoid their food chain contamination and possible human exposure. Highlights: Rare data about foliar uptake of heavy metals & associated biophysiochemical changes. This study shows the effect of foliar PbO-NPs on physiochemical changes in spinach. Foliar PbO-NPs caused Pb uptake in leaves (42 μg g −1 ) and root (4.5 μg g −1 ) of spinach. Foliar PbO-NPs show possible carcinogenic and non-carcinogenic health risks in human. Air pollution of metal-PM need to be monitored regularly to avoid food contamination.