Microsomal activation, and SH-SY5Y cell toxicity studies of tremetone and 6-hydroxytremetone isolated from rayless goldenrod (Isocoma pluriflora) and white snakeroot (Agertina altissima), respectively. (March 2020)

• Record Type: Journal Article
• Authors: Green, Benedict T.; Lee, Stephen T.; Davis, T. Zane; Welch, Kevin D.
• Is Part Of: Toxicon. Volume 5(2020)
• Publication Date: 2020-03
• Subjects: Tremetone -- White snakeroot -- Ageratina altissima -- Rayless goldenrod -- Eupatorium rugosum
• DOI: 10.1016/j.toxcx.2019.100018
• Languages: English
• ISSNs: 2590-1710

Abstract

Abstract: This research compared the cytotoxic actions of the benzofuran ketone, tremetone in B16 murine melanoma cells to SH-SY5Y human neuroblastoma cells with an MTT assay. Tremetone was not cytotoxic in B16 cells. In SH-SY5Y cells, concentration-dependent tremetone cytotoxicity occurred without microsomal activation. No cytotoxicity was observed with 6-hydroxytremetone. This suggests that SH-SY5Y cells are a better model for the cytotoxic actions of tremetone and that tremetone is toxic without microsomal activation. Highlights: Tremetone was not cytotoxic in B16 cells. In SH-SY5Y cells, concentration-dependent tremetone cytotoxicity occurred without microsomal activation. 6-hydroxytremetone was not cytotoxic in SH-SY5Y cells.