Localization of Pasteurella multocida antigens in the brains of pigs naturally infected with Pasteurellosis revealing a newer aspect of pathogenesis. (March 2020)

• Record Type: Journal Article
• Authors: Sahoo, Monalisa; Baloni, Suraj; Thakor, Jigarji Chaturji; M, Dinesh; Bhutediya, Jitendra; Qureshi, Salauddin; Dhama, Kuldeep; Dubal, Zunzar Baburao; Singh, Karampal; Singh, Rajendra
• Is Part Of: Microbial pathogenesis. Volume 140(2020)
• Publication Date: 2020-03
• Subjects: Brain -- Neurovirulence -- Pasteurella multocida -- Pathology -- Pigs -- Virulence genes; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.103968
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Pasteurella multocida is an economically important respiratory pathogen of pigs confronting swine industry worldwide. Despite extensive research over the decades, its pathogenesis is still poorly understood. Recent reports have demonstrated the nervous system affection as a newer aspect of pathogenesis by Pasteurella multocida type B:2 in Haemorrhagic Septicemia, but there are no reports of the involvement of nervous system by P. multocida in pigs. Therefore, the study was aimed to explore the neurovirulence of Pasteurella multocida in naturally infected pigs. A total of 15 brains were collected from the natural cases of pig mortality suggestive of Pasteurellosis. Grossly, the leptomeninges were markedly congested and brains were oedematously swollen. Histologically, there was moderate to severe fibrinohaemorrhagic and mononuclear cells exudates present in the leptomeningeal tissue and cerebrospinal spaces. Similar vascular inflammatory lesions (perivascular and perineuronal) along with gliosis, neuronal degeneration and necrosis were noted in various subanatomical sites of the brain (cerebrum, cerebellum, brainstem and spinal cord). The culture and biochemical tests showed the presence of P. multocida within the brain tissue. P. multocida type specific antibody staining in the brain tissues revealed intense distribution of antigens in the inflammatory exudates of meningeal vessels, neurons, glial cells and endothelial cells of the blood vessels contributing its association with neuropathological lesions. Pasteurella multocida specific PCR amplification of capsular polysaccharide gene yielded 460 bp and multiplex PCR showed the involvement of capsular serogroups A &D. All the isolates showed the presence of 10 genes for virulence factors. The disease confirmation of both serotypes was proven by Koch's postulates using Swiss albino mice. Further, histopathological brain lesions along with the immunohistochemical detection of bacterial antigens were corroborated with natural cases of P. multocida as described above. To the best of our knowledge, we first time report the neuroinvasion of P. multocida in naturally infected pigs. Highlights: Pasteurella multocida is the established cause of pneumonia and sepsis in animals, including pigs. Besides classical lesions of pneumonia, this pathogen showed hallmarks lesions of meningoencephalitis in the brain of pigs. The immunolocalization of P. multocida antigens, bacterial isolation along with the genomic detection in the brain reveal its neurovirulence. The successful establishment of the disease in Swiss albino mice further confirmed its neuropathogenic potential.